Ion Transport in Isolated Rabbit Ileum

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Ion Transport in Isolated Rabbit Ileum

The transmural potential difference, short-circuit current, and Na fluxes have been investigated in an in vitro preparation of isolated rabbit ileum. When the tissue is perfused with a physiological buffer, the serosal surface is electrically positive with respect to the mucosal surface and the initial potential difference in the presence of glucose averages 9 mv. Unidirectional and net Na flux...

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Ion Transport in Isolated Rabbit

The addition of actively transported sugars to the solution bathing the mucosal surface of an in vitro preparation of distal rabbit ileum results in a rapid increase in the transmural potential difference, the short-circuit current, and the rate of active Na transport from mucosa to scrosa. These effects arc dependent upon the active transport of the sugar per se and arc independent of the meta...

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Effect of Inhibitors on Alanine Transport in Isolated Rabbit Ileum

The effects of metabolic inhibitors and ouabain on alanine transport across rabbit ileum, in vitro, have been investigated. Net transport of alanine and Na across short-circuited segments of ileum is virtually abolished by cyanide, 2,4-dinitrophenol, iodoacetate, and ouabain. However, these inhibitors do not markedly depress alanine influx from the mucosal solution, across the brush border, int...

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The effects of electrical field stimulation and tetrodotoxin on ion transport by the isolated rabbit ileum.

To determine whether intramural nerves affect intestinal ion transport, we studied the effect of electrical field stimulation (EFS) on the movement of ions across isolated rabbit ileum. EFS increased the transmural electrical potential difference and the short circuit current (Isc), caused C1 secretion, and reduced conductance, but did not alter fluxes of Na or the residual current (JRnet). The...

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ژورنال

عنوان ژورنال: Journal of General Physiology

سال: 1964

ISSN: 1540-7748,0022-1295

DOI: 10.1085/jgp.47.3.567